An improved multiplex PCR for diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis
نویسندگان
چکیده
منابع مشابه
Diagnosis of Mycoplasma hyopneumoniae.
Mycoplasma hyopneumoniae, the cause of enzootic pneumonia, remains an important pathogen in the swine industry. This small, complex organism colonizes the ciliated cells of the respiratory tract, resulting in little exposure to the immune system. Confirming the presence of M. hyopneumoniae, as well as identifying its role in respiratory disease and pneumonia, remains challenging to the veterina...
متن کاملDiagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis infections in pigs by PCR amplification of the p36 and p46 genes.
The genome of Mycoplasma hyopneumoniae encodes several immunodominant proteins, including a cytosolic protein (p36), three membranous proteins (p46, p65, and p74), and an adhesin (p97). Cross-reactions with M. flocculare and M. hyorhinis reduce the specificity of conventional serological detection methods. However, certain antigenic determinants of the p36 and p46 proteins have been shown to be...
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Swine mycoplasmas are acknowledged for their potential to contribute to several swine disease complexes. In addition, mycoplasma-associated arthritis has recently been recognized as a re-emergent issue in the swine industry. Reports from Veterinary Diagnostic Laboratories suggest an increasing incidence of cases occurring in the major regions of swine production in the U.S. Although previous st...
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The belief that rheumatoid arthritis may be of infectious origin has led us to studies of mycoplasmal arthritidies of swine as a potential animal model (1). In parallel with the previously reported study of Mycoplasma hyorhinis arthritis in conventional Yorkshire swine, a pilot study of the disease in a miniature strain of swine was undertaken on the grounds that a susceptible miniature breed w...
متن کاملGenes involved in translation of Mycoplasma hyopneumoniae and Mycoplasma synoviae
This is a report on the analysis of genes involved in translation of the complete genomes of Mycoplasma hyopneumoniae strain J and 7448 and Mycoplasma synoviae. In both genomes 31 ORFs encoding large ribosomal subunit proteins and 19 ORFs encoding small ribosomal subunit proteins were found. Ten ribosomal protein gene clusters encoding 42 ribosomal proteins were found in M. synoviae, while 8 cl...
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ژورنال
عنوان ژورنال: Korean Journal of Veterinary Research
سال: 2012
ISSN: 2466-1384,2466-1392
DOI: 10.14405/kjvr.2012.52.1.039